Download Advances In Biochemical Engineering Biotechnology Plant by J.-J. Zhong PDF

By J.-J. Zhong

The hot achievements in engineering reports on plant mobilephone cultures are reviewed, incorporated are the gasoline focus results and bioprocess integration for the improved productiveness of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein construction from genetically changed plant cells are brought. Large-scale plant micropropagation through somatic embryogenesis and bushy roots is mentioned for effective propagation of desease-free, genetically uniform and large quantities of vegetation in vitro in giant quantities. Characterization and alertness of bushy plant roots endowed with photosynthetic features can also be coated during this specified quantity.

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5. 2001 10:14 Uhr Seite 51 (Schwarz/Process Black Bogen) Gas Concentration Effects on Secondary Metabolite Production by Plant Cell Cultures 51 thought to carry the signal from the elicited plant to the surrounding unelicited plants. Elicitation of plant cell suspension cultures is normally carried out by addition of a solution of the elicitor. Indeed, for investigators without the means for mixing gases and supplying the mixture to the plant cell culture, application of the plant hormone ethylene is normally conducted by addition of a dilute ethephon solution to the plant suspension culture.

55 References . . . . . . . . . . . . . . . . . . 59 1 Introduction Plants provide a wide variety of biochemicals useful to humanity. Their uses include medicinal compounds, flavors, fragrances and agricultural chemicals. A number of investigators have studied the use of plant cells in culture, rather than whole plants, as sources of some of the more valuable organic compounds. Before such processes can become a viable manufacturing option, a great deal more must be learned about the optimum conditions for growth and productivity of cells in culture.

2 Specific production rate tends to be lower. For example, despite several years of optimization studies, volumetric productivity of shikonin by suspension cultures of L. 1 gproduct l–1 d–1 [23]. C. Linden et al. 2 g l–1 d–1 of penicillin [24]. 3 Plant cells may store their products in vacuoles rather than secrete them into the medium. 4 Plant cells are much more sensitive to shear forces than are bacteria or yeast cells, requiring much gentler aeration and agitation. The medium in contact with cultured plant cells must consist of a large number of components for growth to occur.

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