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Additional info for Advances in Chromatography: Volume 42
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Factors such as mobile phase ﬂow ﬂuctuations, temperature gradients or changes, and column degradation over time contribute to this variation, and ensure that no two chromatographic separations ever have exactly the same time axis, even though their overall run times may be identical. For example, in GC, several factors lead to variations in retention time for a single analyte over a series of many chromatographic runs. The reasons for variation include temperature and ﬂow ﬂuctuations between runs or during a run, matrix effects between samples, and stationary phase composition changes between columns (as well as column degradation over time).
Mayﬁeld, P. R. Kromann, and A. Faruque, Anal. , 67: 3846 (1995). 46. B. K. Lavine, A. J. Moores, H. T. Mayﬁeld, and A. Faruque, Anal. , 31: 2805 (1998). 47. B. K. Lavine, A. J. Moores, H. Mayﬁeld, and A. Faruque, Microchem. , 61: 69 (1999). 48. B. K. Lavine, J. Ritter, A. J. Moores, M. Wilson, A. Faruque, and H. T. Mayﬁeld, Anal. , 72: 423 (2000). 49. B. J. Prazen, C. A. Bruckner, R. E. Synovec, and B. R. Kowalski, J. Microcol. , 11: 97 (1999). 50. A. Felinger, in Advances in Chromatography (P.