Download Aging Methods and Protocols by Yvonne A. Barnett, Christopher R. Barnett PDF

By Yvonne A. Barnett, Christopher R. Barnett

Getting older tools and Protocols offers a few state of the art protocols, defined through well-known specialists of their box, that are getting used to extra our realizing of the getting older strategy. those certain biochemical, actual and molecular biology strategies are mixed to supply an exceptional source for researchers and scientists from a wide selection of disciplines. additionally incorporated is a presentation of 2 case experiences that target the position of nutritional restrict in lifestyles span extension, in addition to how you can determine and make the most of transgenic animals for the elucidation of the molecular facets of getting older. This booklet is a useful resource of cutting-edge learn protocols with large clinical applicability and may curiosity medical researchers, clinicians concerned inIn this paintings, specialists element key biochemical, analytical, and molecular recommendations for the research of growing older on the mobile, tissue, organ, and entire process degrees.

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Natl. Acad. Sci. USA 88, 11,012–11,016. 43. Cristofalo, V. , Doggett, D. , Brooks-Frederich, K. , and Phillips, P. D. (1989) Growth factors as probes of cell aging. Exp. Gerontol. 24, 367–374. 44. , Phillips, P. , and Cristofalo, V. J. (1986) The effect of dexamethasone on epidermal growth factor binding and stimulation of proliferation in young and senescent WI38 cells. Exp. Cell Res. 164, 408–414. 45. , and Cristofalo, V. J. (1993) Effect of the tumor promotor phorbol 12-myristate 13-acetate (PMA) on proliferation of young and senescent WI-38 human diploid fibroblasts.

2. 24 g of KH2PO4 in 900 mL of H2O with magnetic stirring. 4 with HCl, the volume adjusted to 1 L, and the solution is autoclaved for 20 min at 121°C. 3. Emulsion: Kodak NTB-2 emulsion is purchased in a lightproof container. The emulsion is stored at 4°C (see Note 6). 4. Developer and Fixer a. Kodak D-19 developer is purchased in packets that make 1 gal when reconstituted. The entire packet is used at one time and the solution is stored in a brown bottle in the dark. The developer remains useable for 1–3 mo.

Biophys. Res. Commun. 226, 517–523. 85. Millis, A. , McCue, H. , and Martini, H. (1992) Differential expression of metalloproteinase and tissue inhibitor of metalloproteinase genes in aged human fibroblasts. Exp. Cell Res. 201, 373–379. 86. Pignolo, R. , Cristofalo, V. , and Rotenberg, M. O. (1993) Senescent WI-38 cells fail to express EPC-1, a gene induced by young cells upon entry into the G0 state. J. Biol. Chem. 268, 8949–8957. 87. , Allen, R. , Rottenberg, M. , and Cristofalo, V. J. (1999) Expression of EPC-1 in human skin fibroblasts derived from donors of different ages.

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